Journal: Nonlinear Biomedical Physics

Article Title: Evidence of a pharmacodynamic EEG profile in rats following clonidine administration using a nonlinear analysis

doi: 10.1186/1753-4631-5-4

Figure Lengend Snippet: Sleep stage scoring principle

Article Snippet: EEG and EMG data were continuously sampled at 250 Hz and the spectral upper limit was set at 40 Hz, with Data Sciences International hardware and software for 12 hours immediately following administration of drug at light onset.

Techniques:

Journal: ACS chemical neuroscience

Article Title: A Rodent Model of Traumatic Stress Induces Lasting Sleep and Quantitative Electroencephalographic Disturbances

doi: 10.1021/cn500342u

Figure Lengend Snippet: Experimental design for EEG studies and tissue collection. In cohort 1, continuous EEG, EMG, and temperature data were telemetrically recorded from chronically implanted rats throughout successive 24 h light-dark cycles (ON, 6:00 am; OFF, 6:00 pm) before (BL) and several days after (days 0, 1, 2, and 7) either single prolonged stress (SPS) or SHAM treatment. Both treatments were performed within the first 6 h of the light phase on day 0, during which recording was not possible; EEG data from this day was reinitiated when each animal was returned to its home cage. In cohort 2, nonimplanted aged-matched rats underwent either SPS or SHAM treatment. SPS rats were sacrificed either 1 h (day 0), 1 day (day 1), or 7 days (day 7) later; SHAM rats were sacrificed 7 days later.

Article Snippet: Sleep Staging EEG, EMG, and temperature data were collected with Dataquest A.R.T.

Techniques:

Journal: The Journal of Neuroscience

Article Title: Dynamic Network Activation of Hypothalamic MCH Neurons in REM Sleep and Exploratory Behavior

doi: 10.1523/JNEUROSCI.0305-19.2019

Figure Lengend Snippet: Deep-brain imaging of MCH neurons. A, Schematic of transfection of MCH neurons in MCH-Cre mice with AAV-DIO-GCaMP6 followed by placement of the GRIN lens in region transfected with GCaMP6 (slow or medium). The miniscope is attached to the GRIN lens via a baseplate on the skull. B, Photomicrograph depicts the location of the GRIN lens (outlined in dashed lines) atop the body of GCaMP6s containing neurons in the hypothalamus in a representative MCH-Cre mouse. The brain region containing the GRIN lens was sectioned along the coronal axis of the brain, and tissue containing the GCaMP6s neurons were identified. f, Fornix. Scale bar, 300 μm. C, Immunohistochemistry revealed that GCaMP6s-infected neurons (green) were also immunopositive for MCH. The coronal sections were incubated with the MCH antibody and visualized using a Leica confocal microscope. Scale bar, 80 μm. D, The field of view of the GRIN lens with fluorescence (ΔF/F0) in somata and processes during REM sleep in neurons extracted automatically by PCA-ICA analysis. We have labeled the three neurons (labeled 1, 2, and 3) whose Ca2+ fluorescence is plotted in E. E, GCaMP6s fluorescence (ΔF/F0) in MCH neurons is associated with REM sleep. Ca2+ imaging was performed simultaneously with recording of cortical EEG and EMG activity in the nuchal muscles. Behavioral video recordings were obtained and examined to identify behaviors such as walking, eating, grooming, or eating. Activity in the EEG (depicted as power spectra, 0.3–15 Hz) and the EMG is used to identify wake, NREM, and REM sleep states (labeled as hypnogram). The traces depict the change in fluorescence (ΔF/F) during wake–sleep bouts of the three neurons identified in D. In each neuron, the ΔF/F0 (expressed as a z-score) varies with the wake–sleep state of the animal, with peak fluorescence associated with REM sleep. The hypnogram categorizes the sleep–wake states in the following colors: purple, active wake; blue, quiet wake; green, NREM; yellow, pre-REM sleep; red, REM sleep. F, The same field of view as in D, but this image shows the PCA-ICA extracted neurons (ΔF/F0) while the mouse was engaged in exploring novel objects placed in its home cage. This image shows that some neurons that were evident in REM sleep (D) were also activated during exploratory behavior. However, some neurons in D were not evident during exploratory behavior, indicating selective activation of these neurons during REM sleep (D). Thirty percent of the neurons were activated during REM sleep but not during exploratory behavior, indicating that a subset of MCH neurons is selectively active in REM sleep. G, GCaMP6s fluorescence in MCH neurons while exploring novel objects. The traces are from the same neurons represented in REM sleep (E). Note that the GCaMP6s has a rapid response and a slow rate of decay, which makes it difficult to infer whether the imaged neuron fired as single spikes or in clusters.

Article Snippet: The sleep–wake states were identified based on EEG, EMG and video data (Neuroexplorer; Plexon).

Techniques: Imaging, Transfection, Immunohistochemistry, Infection, Incubation, Microscopy, Fluorescence, Labeling, Activity Assay, Muscles, Activation Assay

Journal: NeuroImage : Clinical

Article Title: Abnormal functional corticomuscular coupling after stroke

doi: 10.1016/j.nicl.2018.04.004

Figure Lengend Snippet: Group-average corticomuscular coherence between EEG and EMG of BB and DT muscles, for each muscle, A and B shows its coherence with the scalp areas for the synkinetic patients, C and D for separate patients and E and F for controls. For A, B, C, D, E and F, the y-axis is frequency, the x-axis is time, and the color bar indicates the level of coherence (red, higher level; blue, lower level). G, H, I and J show the mean coherence values based on EEG and EMG signals from 2.5 to 4.5 s and from 5.5 to 7.5 s, corresponding roughly from the beginning to the end of reaching movement. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: EEG and EMG data were recorded synchronously by 64 channels NeuroScan system (Synamp2, Compumedics Inc., Charlotte, NC, USA), and the motion data was also synchronously by the Perception legacy system (TM-SI-8-STD, NoitomTechnology Ltd., Beijing, China).

Techniques: Muscles